Ammonia, nitrate and nitrite Open Colorimeter assays for water quality testing

This week we finished testing and implementing three new water quality tests for the Open Colorimeter. These assays are ammonia, nitrate and nitrite. We have had quite a few requests for these tests from researchers and hobbyists. They are important water parameters to measure in aquariums, ponds and other wildlife habitats. Anyone with an aquarium should be routinely monitoring these 3 parameters as they are critical to fish health. For the colorimetric assays, we used the popular API test kits. These are easy to use, fairly inexpensive and you can buy them online or from most pet or aquarium stores. They typically range around $10-15 each for around 90-150 tests.

Instructions for using these tests:

• Take a 5mL sample of water to be tested e.g. river or aquarium water
• Add drops of the test kit per instructions (e.g. for nitrite, add 5 drops of test solution) and wait for color to develop
• Visually match the color to the chart provided with the kit

This last step where users have to visually match their color to a chart is not an actual quantitative measurement, but gives you a range of concentrations. This is not ideal obviously, especially for monitoring changes in concentrations or accurately comparing samples.

Quantitative measurements using the Open Colorimeter

Open Colorimeter

🎃 Shipping dates: We expect to start shipping the week of Oct. 31st! The Open Colorimeter is a portable and customizable colorimeter for measuring absorbance, transmittance and concentration. The cuvette holder and enclosure are made from 3D-printed black nylon material and the electronics are prog…

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We can vastly improve water quality measurements by using the Open Colorimeter to quantitatively measure the amount of ammonia, nitrate and nitrite in water sample using these same affordable and easy to use test kits. The test color is developed as described above, but instead of the chart method, the sample is placed into the Open Colorimeter to measure absorbance and concentration. This week we have finished testing the ammonia, nitrate and nitrite assays and these tests will be included with all Open Colorimeter's we ship! Below are images of the tests and links to each of the tutorials for these assays.

Ammonia

• Colorimetric assay uses the API ammonia test kit
• Measures from 0-8ppm ammonia (NH₃)
• Tutorial: https://blog.iorodeo.com/ammonia-api-colorimetric-assay/

Ammonia-API colorimetric assay

Colorimetric assay for measuring ammonia levels using API test kit. Range: 0-8ppm

IO Rodeo ResourcesJoanne Long

Nitrate

• Colorimetric assay uses the API nitrate test kit
• Measures from 0-50ppm nitrate (NO₃)
• Tutorial: https://blog.iorodeo.com/nitrate-api-colorimetric-assay/

Nitrate-API colorimetric assay

Colorimetric assay for measuring nitrate levels using API test kit. Range: 0-50ppm

IO Rodeo ResourcesJoanne Long

Nitrite

• Colorimetric assay uses the API nitrite test kit
• Measures from 0-2ppm nitrite (NO₂)
• Tutorial: https://blog.iorodeo.com/nitrite-api-colorimetric-assay/

Nitrite-API colorimetric assay

Colorimetric assay for measuring nitrite levels using API test kit. Range: 0-2ppm

IO Rodeo ResourcesJoanne Long